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Philip Reiner Kensche edited this page Nov 17, 2021
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19 revisions
This plugins contains alignment and quality control related Roddy workflows:
PanCancer alignment workflow for
whole genome sequencing (WGS) and
whole exome sequencing (WES)
Whole genome bisulfite workflow (WGBS) using methylCtools. For tagmentation WGBS and PBAT a modified version is used (described here).
These workflows are basically BWA alignment (bwa mem) workflows with plenty of additional quality control steps.
QC-steps acting on the BAM files are mostly done through piping the input data into multiple QC tools simultaneously to minimize I/O and thus maximize throughput.