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Pelotomaculum-Methanocella propionate syntrophy: curate causal graph#243

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realmarcin merged 1 commit into
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causal/pelotomaculum-methanocella-000190
Jul 22, 2026
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Pelotomaculum-Methanocella propionate syntrophy: curate causal graph#243
realmarcin merged 1 commit into
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causal/pelotomaculum-methanocella-000190

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What

Adds directed downstream causal edges to the four existing ecological_interactions of CommunityMech:000190 (Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture), from an Edison causal-graph run (PaperQA3 causal-edge mode; single source PMID:30038609 / DOI:10.3389/fmicb.2018.01551).

InteractionDownstream carries target + a causal description only (no evidence field) — the supporting evidence stays on the parent interaction nodes, whose existing snippets already validate against the cached abstract. No new evidence snippets or terms were introduced.

Causal wiring (syntrophic feedback loop 1→2→3→1 + negative edge)

From To Sign Basis (abstract-grounded)
Propionate Oxidation Coupled to Methanogenesis Catabolism & Methanogenesis Upregulation + Syntrophic coculture drives the concerted catabolic/methanogenesis transcriptional shift
Catabolism & Methanogenesis Upregulation Formate & Electron Bifurcation Strategy + Upregulated FDH + flavin electron-bifurcation genes activate formate/H2 electron transfer
Catabolism & Methanogenesis Upregulation Signal Transduction & Amino Acid Exchange + Same shift upregulates flagellar signaling + amino-acid exchange
Formate & Electron Bifurcation Strategy Propionate Oxidation Coupled to Methanogenesis + Formate/H2 transfer sustains methanogenesis, thermodynamically pulling propionate oxidation; 2-BES abolishes both
Formate & Electron Bifurcation Strategy Propionate Oxidation Coupled to Methanogenesis Product inhibition: excess formate halts syntrophic activity

All taxa use the record's canonical NCBITaxon ids (110500 / 1175444).

Verification

  • just validate → No issues found.
  • Reference validator behaves identically to prior causal records (no new snippets).
  • Record HTML page regenerated (single file) — downstream edges render.

Continues the causal-graph curation thread (after #226/#229/#230). Provenance bundle under research/communities/…-causal.* (gitignored).

🤖 Generated with Claude Code

Add directed `downstream` causal edges to the four existing
ecological_interactions of CommunityMech:000190, from the Edison
causal-graph run (PaperQA3; PMID:30038609 / DOI:10.3389/fmicb.2018.01551).

Wiring (all grounded in the cited abstract; InteractionDownstream carries
target + causal description, evidence stays on the parent nodes):
- Propionate Oxidation Coupled to Methanogenesis → Catabolism and
  Methanogenesis Upregulation (syntrophic coculture drives the concerted
  catabolic/methanogenesis transcriptional shift).
- Catabolism and Methanogenesis Upregulation → Formate and Electron
  Bifurcation Strategy, and → Signal Transduction and Amino Acid Exchange
  (the upregulation activates FDH/electron-bifurcation transfer and the
  flagellar-signaling / amino-acid-exchange mechanisms).
- Formate and Electron Bifurcation Strategy → Propionate Oxidation Coupled
  to Methanogenesis, twice: a positive edge (formate/H2 transfer sustains
  methanogenesis, thermodynamically pulling propionate oxidation; 2-BES
  abolishes both) and a NEGATIVE product-inhibition edge (excess formate
  halts syntrophic activity).

Forms the syntrophic feedback loop 1->2->3->1 with a negative regulatory
edge. Regenerate the record's HTML page (single file). Schema validates;
no new evidence snippets or terms introduced.

Co-Authored-By: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
Copilot AI review requested due to automatic review settings July 22, 2026 02:11

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Pull request overview

This PR curates Edison causal-graph output into CommunityMech:000190 by adding directed downstream causal edges to existing ecological_interactions in the Pelotomaculum–Methanocella propionate syntrophy record, and regenerates the corresponding HTML page so the downstream wiring is reflected in rendered docs.

Changes:

  • Add downstream entries (target + causal description) to 3 existing ecological_interactions in CommunityMech:000190.
  • Regenerate the community’s HTML page to display downstream effects.

Reviewed changes

Copilot reviewed 2 out of 2 changed files in this pull request and generated 1 comment.

File Description
kb/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.yaml Adds curated causal downstream edges (with descriptions) to existing interaction nodes.
docs/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.html Regenerated output to show “Downstream Effects” sections for the updated interactions.

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Comment on lines +825 to +836
<div class="interaction-flow">
<strong>Downstream Effects:</strong>

<div class="flow-item">
<span class="flow-arrow">→</span> Propionate Oxidation Coupled to Methanogenesis
</div>

<div class="flow-item">
<span class="flow-arrow">→</span> Propionate Oxidation Coupled to Methanogenesis
</div>

</div>
@realmarcin
realmarcin merged commit 3a4fc7c into main Jul 22, 2026
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@realmarcin
realmarcin deleted the causal/pelotomaculum-methanocella-000190 branch July 22, 2026 02:17
realmarcin added a commit that referenced this pull request Jul 22, 2026
… resolved (#244)

Add CommunityMech:000190 to the causal-graph "done" list (~59/304), and
replace the Edison-auth blocker note with the resolution: the .env key was
refreshed and authenticates, but the shell profile's stale
EDISON_PLATFORM_API_KEY still shadows/403s it, so run causal jobs with
`env -u EDISON_PLATFORM_API_KEY` until that export is fixed.

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
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2 participants