diff --git a/docs/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.html b/docs/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.html
index 02080172..f78d54ca 100644
--- a/docs/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.html
+++ b/docs/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.html
@@ -409,6 +409,7 @@
Pelotomaculum-Methanocella Propionate RNA-Seq Coculture
+
@@ -655,6 +656,15 @@ Propionate Oxidation Coupled to Methanogenesis
+
+
Downstream Effects:
+
+
+ → Catabolism and Methanogenesis Upregulation in Coculture
+
+
+
+
Evidence
@@ -726,6 +736,19 @@ Catabolism and Methanogenesis Upregulation in Coculture
+
+
Downstream Effects:
+
+
+ → Formate and Electron Bifurcation Strategy
+
+
+
+ → Signal Transduction and Amino Acid Exchange
+
+
+
+
Evidence
@@ -799,6 +822,19 @@ Formate and Electron Bifurcation Strategy
+
+
Downstream Effects:
+
+
+ → Propionate Oxidation Coupled to Methanogenesis
+
+
+
+ → Propionate Oxidation Coupled to Methanogenesis
+
+
+
+
Evidence
diff --git a/kb/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.yaml b/kb/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.yaml
index fec452fa..82c62081 100644
--- a/kb/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.yaml
+++ b/kb/communities/Pelotomaculum_Methanocella_Propionate_RNASeq_Coculture.yaml
@@ -149,6 +149,13 @@ ecological_interactions:
term:
id: GO:0015948
label: methanogenesis
+ downstream:
+ - target: Catabolism and Methanogenesis Upregulation in Coculture
+ description: >
+ Establishing the syntrophic coculture (versus monoculture) drives the
+ concerted transcriptional upregulation of catabolic genes — the
+ methylmalonyl-CoA pathway in P. thermopropionicum and methanogenesis
+ genes in M. conradii.
evidence:
- reference: PMID:30038609
supports: SUPPORT
@@ -185,6 +192,17 @@ ecological_interactions:
term:
id: GO:0015948
label: methanogenesis
+ downstream:
+ - target: Formate and Electron Bifurcation Strategy
+ description: >
+ The catabolic upregulation includes formate dehydrogenase and flavin-based
+ electron bifurcation/confurcation genes in both partners, activating
+ formate as an alternate interspecies electron carrier alongside H2.
+ - target: Signal Transduction and Amino Acid Exchange
+ description: >
+ The same coculture transcriptional shift upregulates flagellum-induced
+ signal transduction and amino-acid exchange that accompany syntrophic
+ growth.
evidence:
- reference: PMID:30038609
supports: SUPPORT
@@ -222,6 +240,19 @@ ecological_interactions:
term:
id: GO:0044419
label: biological process involved in interspecies interaction between organisms
+ downstream:
+ - target: Propionate Oxidation Coupled to Methanogenesis
+ description: >
+ Interspecies formate and H2 transfer to M. conradii sustains
+ hydrogenotrophic methanogenesis, keeping the shared electron-carrier
+ partial pressure low and thermodynamically pulling P. thermopropionicum
+ propionate oxidation; inhibiting methanogenesis with 2-BES abolished both
+ methane production and propionate oxidation.
+ - target: Propionate Oxidation Coupled to Methanogenesis
+ description: >
+ NEGATIVE (product inhibition) — accumulation of excess formate inhibits
+ syntrophic activity and halts propionate oxidation, so formate transfer
+ must stay balanced by continuous methanogenic consumption.
evidence:
- reference: PMID:30038609
supports: SUPPORT